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Baseline Levels of Interleukine-6, Tumor-Necrosis Factor-Alpha and C-Reactive Protein in Treatment Naive Human Immunodeficiency Virus Infected Patients-A Study from a Tertiary-Care Hospital in Eastern India

[ Vol. 6 , Issue. 2 ]

Author(s):

Anindita Debnath, Madhuchhanda Mandal, Sayantani Choudhuri, Malay K. Saha and Subhasish Kamal Guha   Pages 124 - 132 ( 9 )

Abstract:


Background: Inflammatory cytokines play a major role in pathophysiology, prognosis and progression in HIV infection. Studies on pre-ART levels of different pro-inflammatory and coagulation markers have shown predictive role on progression and mortality in HIV. IL-6, CRP has also been shown to be correlated with HIV-RNA levels as disease progresses.

Objective: Biomarkers of inflammation are of increasing interest as predictors of morbidity and mortality in HIV infected patients. Though India is a major contributor to HIV-related mortality in Asia and Pacific zone, studies on inflammatory markers predictive of progression of HIV disease, on population from the Indian sub-continent are few. In this study, we observed the baseline levels of inflammatory cytokines among treatment naïve HIV positive subjects in respect to healthy volunteers.

Materials and Methods: Serum levels of IL-6, TNF-α and CRP in 130 antiretroviral naive, HIVpositive individuals were estimated using sandwich ELISA, along with routine CD4 cell count, plasma HIV-1 viral load and biochemical tests for clinical assessment. Cytokine levels between ‘study’ and ‘control’ arms (30 age & gender matched HIV-negative healthy volunteers) were compared. Independent unpaired t-test was done using Graph Pad Prism 6.0 to analyze differences statistically.

Results: Baseline cytokine levels were significantly high (p ≤ 0.05) in ARV naive HIV-positive subjects compared to healthy controls. Cytokine levels among treatment naive patients also correlated with severity of immunosuppression.

Conclusion: Biomarkers of inflammation (IL-6, TNF-α) and CRP were found to be high in ARV naïve HIV-positive subjects, enrolled at an Eastern Indian tertiary hospital. Extensive studies on baseline inflammatory profile of patients may be predictive of disease progression, treatment response and mortality.

Keywords:

CD4 cell count, C-reactive protein (CRP), inflammatory markers, interleukine-6 (IL-6), plasma viral load (PVL), tumor necrosis factor-α (TNF-α).

Affiliation:

Department of Laboratory Medicine, Calcutta School of Tropical Medicine, Kolkata, West Bengal, Department of Tropical Medicine, Calcutta School of Tropical Medicine, Kolkata, West Bengal, Department of Laboratory Medicine, Calcutta School of Tropical Medicine, Kolkata, West Bengal, Virology, Molecular HIV Lab, National Institute of Cholera and Enteric Diseases, Kolkata, West Bengal, Department of Tropical Medicine, Calcutta School of Tropical Medicine, 108-C.R. Avenue, Kolkata- 700073, West Bengal



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